WebApr 13, 2024 · The KanMX6 fragment was amplified from the pFA6a-GFP(S65T)-kanMX6 plasmid. S. cerevisiae BY4741 competent cells were transformed with the amplified KanMX6 fragment using yeast transformation kit. Transformants were plated on YPD agar plates containing 600 ng/mL G418 and cultured at 30ºC for one week. Obtained colonies were … WebFeb 29, 2016 · Chiu等把来自拟南芥菜的干旱诱导型启动子GFP(S65T) 重组转化烟草并得到转基因植株。当水分逐渐亏缺时，绿色荧光开始出现在叶片 的表皮、叶肉和保卫细胞中，而且保卫细胞和腺毛中荧光最强。 ... GFP可以用手提式长波紫外灯观察，也可以 用荧光显微镜 …
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WebGreen fluorescent protein (GFP) has emerged as a powerful new tool in a variety of organisms. An engineered sGFP(S65T) sequence containing optimized codons of highly … WebProperty; Application areas: Can be used in electronics,communications,high-frequency heating industrial equipment and measurement, inspection and test equipment such as …
WebOct 16, 2012 · S65T is considered essential for suppressing the 395 nm excitation peak through modulation of the ionized state of nearby Glu222, whilst the F64L mutation is … WebGFP (S65T) (N terminal on insert) yeast ADH1 terminator (N terminal on insert) A. gossypii translation elongation factor 1a gene promoter (N terminal on insert) A. gossypii …
WebApr 18, 2008 · The green fluorescence protein used in these plants is referred to as GFP S65T, a “red shifted” version of the original jellyfish GFP [14-16]. This GFP was engineered to have a peak excitation at 488 nm and peak fluorescence at 512 nm, and has been deployed widely in the laboratory environment as a reporter for plants [1, 17, 18]. WebFeb 1, 1996 · Mutagenesis of the clone encoding S65T, to shift its wavelengths further to the red, yielded a variant with two further substitutions, M153A/K238E. This GFP variant had the longest-wavelength excitation maximum yet described, 504 nm, as compared with 490 nm for S65T (Table 1). Surprisingly, the emission peak hardly changed (514 nm as …
WebTemperature: FPs maturation times and fluorescent intensity can be affected by the temperature. For instance, enhanced GFP (EGFP) was optimized for 37°C, and is …
WebSep 1, 2007 · Relative to the wild-type GFP, Emerald GFP was originally reported to have 5 amino acid (aa) changes (S65T, S72A, N149K, M163T, and I167T) . In a subsequent review, an additional amino acid substitution (F64L) has also been listed [5] ; furthermore, a commercial source of Emerald GFP (EmGFP) also cites the F64L mutation, and in … churchill statue halifaxWebApr 13, 2024 · The oligonucleotide primers used for strain and plasmid construction in this study are listed in Table S1. A previously described recombinant polymerase chain reaction (PCR) method (Krawchuk and Wahls 1999) was used to construct the tfs1::kanMX6, mca1::kanMX6, moc3::kanMX6, tfs1-GFP(S65T)-natMX6, and tfs1-13Myc-natMX6 strains. devonshire collection pulling sleigh bedWebNov 1, 2002 · Detection of transient expression of EGFP or GFP-S65T was carried out using a compound microscope equipped with an attachment for fluorescence observations (model E600, Nikon, Japan). Observations and image captures were carried out at 2× magnification using a Plan-Flor objective. devonshire commons columbus ohioWebNov 12, 1998 · GREEN FLUORESCENT PROTEIN (65-67 REPLACED BY CRO, S65T SUBSTITUTION, Q80R) PDB DOI: 10.2210/pdb1EMG/pdb Classification: … churchill state school facebookhttp://www.cnrongcheng.com/Article-3324787.html churchill statue in edmotonWeb豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座,心理学等数亿实用 ... devonshire commercial property limitedWebDec 13, 2024 · Also Known As UAS-GFP, UAS-GFP S65T, UAS-GFP.S65T, UAS-GFP S65T, UAS-GFP (S65T), UAS-eGFP, UAS-gfpS65T, UAS-S65T-GFP, UAS-cGFP, UAS … churchill statues uk