WebDec 1, 2024 · The neurogenic niche of the subventricular zone (SVZ) in adult brain tissue takes the form of a pinwheel-like cytoarchitectural structure, with mono-ciliated astrocytes displaying neural stem cell ... WebJan 11, 2024 · Moreover, addition of LPA alone to the SFM was sufficient to stimulate proliferation of astrocytes, and partially recapitulate the difference observed between ciliated and deciliated cells in the ...
Frontiers Organized Neurogenic-Niche-Like Pinwheel Structures ...
WebMar 2, 2024 · Primary cilia are centriole-derived sensory organelles that are present in most mammalian cells, including astrocytes and neurons. Evidence is emerging that astrocyte and neuronal primary cilia demonstrate a dichotomy in the mature mouse brain. However, it is unknown how astrocytic and neuronal primary cilia change their morphology and … WebOct 15, 2024 · Morphine induces frequency and length of primary cilia in astrocytes. (A) Scanning electron microscopy image displaying a solitary primary cilium projecting from … greenspring community.com
Ependymal cells: biology and pathology - PubMed
WebDec 20, 2024 · The cilia of astrocytes within the SVZ make contact with the ventricular fluid, and these mono-ciliated cells confer the pinwheel architecture to the ventricular surface … For adult astrocyte cultures, C57BL/6 J mice at an age of 100–140 days were used, whilst neonatal cells were taken from p 4–8 C57BL/6 J pups. Female and male mice were mixed for all analyses. Mice were group housed under specific pathogen-free conditions on a 12 h light/dark cycle, and food and water were … See more Adult astrocytes were isolated using magnetic-activated cell sorting (MACS; Miltenyi Biotec) according to the manufacturer’s protocol. In brief, mice were anesthetized with Isoflurane, euthanized with … See more Astrocytes were differentiated from embryonic stem cells derived from C57Bl/6 N mice (mESCs; GSC-5003, MTI-GlobalStem) using a previously published protocol16. mESCs were first differentiated into … See more Neonatal astrocytes were isolated by using MACS from p 4–8 C57BL/6 J mice. Cerebral tissue was isolated, meninges removed and brains of two mice pooled in a C-tube for tissue … See more Cell culture plates (24-well or 96-well as indicated; BD Biosciences) were prepared two days prior to astrocyte isolation. First, wells were coated with 20 µg/ml poly-l-lysine (PLL) (Sigma, 2636-25MG) in PBS overnight at 37 °C, … See more WebFeb 23, 2024 · Astrocyte. Astrocytus. 1/2. Astrocytes are the largest and most abundant type of supporting (glial) cells in the central nervous system (CNS). Together with … green spring corona test anwendung